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Goods and Services Tax, which subsumed more than a dozen of levies, stood as the highest contributor for the government in terms of collecting revenues. Hailed as the biggest tax reform since Independence, GST single-handedly contributed 32.8 per cent of the total revenues in 2017-18, data from th(Added: Fri Feb 02 2018)

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CHO cells with at least 80% confluence were transfected by Lipofectamine 2000 (Invitrogen, Carlsbad, CA) with 3 ��g of TWIK-1 plasmids and 1 ��g of pEGFP plasmids and studied 24?h later. GFP expression was used to identify effectively transfected CHO cells. Whole-cell patch-clamp recordings (Added: Fri Feb 02 2018)

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