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Various Frightening But Also Constructive Roscovitine Ideas

Added: (Wed Oct 10 2018)

Pressbox (Press Release) - 2?mM potassium and 0?mM, 1?mM, 2?mM or 4?mM methylglyoxal. Survival in all assays was assessed by plating serial dilutions on 7H11 and counting of cfu. J774 macrophages were detached from https://www.selleckchem.com/ tissue culture vessels, resuspended in DMEM without phenol red, and seeded at 1?��?105 per well in 96-well black-walled optical plates. BCG Pasteur wild type and BCG��kef were washed in PBS/0.05% Tween-80, resuspended in DMEM without phenol red, and added to the cells at an moi of 10. Lucigenin (Sigma-Aldrich) was added to a final concentration of 250 micromolar, and the luminescence of the plate was read at 20?min intervals for 60?min using a Wallac plate reader (Perkin-Elmer). Survival of individual mutants in macrophages or during in vitro stresses and frequencies of colocalization with Lysotracker obtained by confocal microscopy were analysed by Student's t-test. One asterisk indicates P? Submitted by:

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